Haynes Lab:Notebook/Inteins/2013/02/13

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Entry title Redid the restriction/digest on 9/10 Incubated for 20 mins. Added 10 ul of the ladder, and 30 ul of the dna Ran gel for more than 30 mins to run gel KAH 9 is 687 bp KAH 10 is NLS Vo120 is 2400 bp for backbone Cut out 10B for extraction Do another restriction/digest for longer with the enzymes http://openwetware.org/wiki/Image:2013-02-13_gel.jpg