Griffin:Zinc-Fixation
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Zinc Fixation Protocol
Zinc-Fixative:
- 1000 mL 0.1M Tris buffer, pH 7.4
- 0.5 g Calcium Actate
- 5.0 g Zinc Acetate
- 5.0 g Zinc Chloride
Mix to dissolve, do not adjust pH (it should be about 6.5-7.0).
I) Place freshly dissected tissue (3 mm or thinner) in Zinc-Fixative and allow to sit at room temperature 6-48 hours.
II) Dehydrate tissues as follows, all at room temperature:
- one hour 70% ethanol
- one hour 95% ethanol
- one hour 100% ethanol
- one hour 100% ethanol
III) Clear tissues as follows, both at room temperature:
- 50 minutes in Xylene
- 50 minutes in Xylene
IV) Follow with paraffin embedding.