Category:In vitro
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Items under In vitro category.
Pages in category "In vitro"
There are 151 pages in this category.
"
"Core Sample" PCR: Amplifying DNA from agarose gels
'
'Round-the-horn site-directed mutagenesis
A
Acrylamide gels
Affymetrix DNA labelling for gene expression arrays
Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
Affymetrix Target Hybridization
Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
Agarose gel electrophoresis
Alm:Agarose gel electrophoresis
Anneal Extend PCR Protocol - for linking 2 pieces of DNA
Annealing and primer extension
Annealing primers
Assembly pcr
B
Beta-galactosidase assay
C
Centrifugal filtration/Nucleic acids
Chromatin Immunoprecipitation
D
DNA Precipitation
DNA Synthesis from Oligos
DNA ligation
Designing primers
Dot Blot
E
Electrophoretic mobility shift assay
Endy:Double stranding oligo libraries
Endy:E. coli Western Blot
Endy:Northern Blot, 32P End-Labeled Probes
Endy:Northern blot, AlkPhos end-labeled probes
Endy:RNA extraction
Endy:Templiphi
Engineering BioBrick vectors from BioBrick parts/DNA ligation
Engineering BioBrick vectors from BioBrick parts/Dephosphorylation
Engineering BioBrick vectors from BioBrick parts/Restriction digest
Etchevers:ChIP francais
Etchevers:Extraction ARN francais
Ethanol precipitation of nucleic acids
Ethanol precipitation of small DNA fragments
F
Free Sulfhydryl Determination
G
Gautier:TUNEL
H
Hop DNA Isolation
I
ISISBio:Protocols/Sortase mediated ligation
ISISBio:Protocols/Sortase mediated ligation/Protein DNA ligation
ISISBio:Protocols/Sortase mediated ligation/Small molecule ligation
ISISBio:Protocols/Sortase mediated ligation/Solid support ligation
ISISBio:Protocols/p11 resin preparation
In vitro transcription with T7 RNA polymerase
J
Jacobs:Protocol Actin Staining
Jacobs:Protocol Adipocyte Differentiation
Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
Jacobs:Protocol Collagen Gel Contraction
Jacobs:Protocol Collagen Gel Contraction 2
J cont.
Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins
Jacobs:Protocol Oil Red O Staining
Jacobs:Protocol Pipetting Exercise
Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
Jacobs:Protocol RNA Agarose Gel
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin
Jamesh008:consensus DNA ligation protocol
K
Keating:Experimental Protocols:HPLC
Knight:Agarose gel electrophoresis
Knight:Annealing and primer extension with Klenow polymerase
Knight:Annealing and primer extension with Taq polymerase
Knight:Centrifuge desalting
Knight:Centrifuge desalting/Zeba columns
Knight:DNA ligation using NEB Quick Ligation Kit
Knight:DNA ligation using T4 DNA ligase
Knight:Electrophoretic mobility shift assay
Knight:In vitro transcription
Knight:Maxiprep
Knight:Micropure EZ and Microcon purification
Knight:Miniprep low copy plasmids
Knight:NuPAGE electrophoresis
Knight:NuPAGE electrophoresis/Fast staining
Knight:NuPAGE electrophoresis/Gel drying
Knight:NuPAGE electrophoresis/Hybrid staining
Knight:NuPAGE electrophoresis/Slow staining
Knight:PhoenIX Maxiprep Kit
Knight:Protein solubility
Knight:Purification of His-tagged proteins
Knight:Purification of His-tagged proteins/Denaturing
Knight:Purification of His-tagged proteins/Denaturing with refolding
Knight:Purification of His-tagged proteins/Native
Knight:RNA electrophoresis
Knight:RNA electrophoresis/Denaturing
Knight:RNA electrophoresis/Native
Knight:Reconstituting primers
Knight:Restriction Digest
Knight:Sequencing DNA
Knight:Site-directed mutagenesis/Multi site
Knight:Site-directed mutagenesis/Single site
Knight:TOPO TA cloning
Knight:TempliPhi
L
Library Generation
Lory:Quick transformant screen
M
MIT:Sequencing BioBrick DNA
Making construction plasmid
Marek: Freeze-down/Thaw
Maxiprep of plasmid DNA from E. coli
Miniprep
Miniprep/GET buffer
M cont.
Miniprep/Kit-free high-throughput protocol
Miniprep/Qiagen kit
N
NanoBio: PCR
Northern Blot, 32P End-Labeled Probes
O
One step 'miniprep' method for the isolation of plasmid DNA
OpenWetWare:New visitor blurb
P
PCR
PCR Overlap Extension
PCR techniques
PNK Treatment of DNA Ends
Phenol/chloroform extraction
Phosphatase treatment of linearized vector
Probe Prep, 32P End-Labeled Probes
Purification of DNA
Purification of His-tagged proteins
Q
Q-PCR
QRT-PCR
QRT-PCR/Single tube
QRT-PCR/Two tubes
Quantitating nucleic acids
R
RNA Half-life
RNA Quality Control
RNA blot (Northern)
RNA electrophoresis
RNA extraction
RNase Protection Assay
Real-time PCR
Reconstituting primers
Restriction digest
S
Sauer:Expression/purification of 35S-Met proteins
Sauer:Lysing E. coli with Lysozymes
Sauer:RNA Purification from E. coli
Sauer:Stitching Genes by PCR
Sequencing DNA
Seth:Quick Transformant Screen
Silver staining
Silver: Coomassie Stain
Silver: Destain
Silver: Lysate for Western
Silver: PCR
Site-directed mutagenesis
Streptavidin purification of DNA fragments
Streptomyces:Protocols/DNA Digest
T
TempliPhi
Topoisomerase I mediated TA cloning
Transformation Protocol for Arabidopsis
Transformation Protocol for Tobacco
U
User:Leprevost/Purification of His-tagged proteins/Denaturing
W
Western blot
Y
Yeast DNA Prep
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