AFM
We deposited the sample (typically 5 µl with ~5 nM DNA origami purified
of excess staple strands) containing rectangles with DNA origami
add-ons on a freshly cleaved mica (grade V1, Ted Pella), incubated for
60 s and then rinsed with 100 µl of buffer contained in the sample
and then two times with 100 µl of MQ water. The sample was dried under
nitrogen stream. Atomic force microscopy measurements were performed on
dried samples in tapping mode on Agilent Technologies 5500 Scanning
Probe Microscope using either silicon cantilevers ARROW-NCR or SSS-NCH
(Nanoworld). Scan rate was generally 1 Hz and below, with engaging
amplitude of 300-400 mV.
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| Figure 49. Vid operating our atomic force microscope |
