Biomod/2011/Slovenia/BioNanoWizards/methafm
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</head> <body> <div id="wrapper"> <div id="headercss" class="container"> <div id="logo"> <div id="logolinknabiomod"> <p><a href="http://openwetware.org/wiki/Biomod/2011"><-- Back to BioMod 2011</a></p> </div> <div id="jebenimeni"> <!-- Start PureCSSMenu.com MENU --> <ul class="pureCssMenu pureCssMenum">
<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards">Home</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>Idea</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/ideastateoftheart">State
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tethers</a></li>
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<li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>Discussion</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/discussion">Discussion</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>Applications</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/applabonchip">Lab-on-a-nanochip</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/appbiosynthteticcompartments">Biosynthetic
compartments</a></li>
<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/appbiosensors">Biosensors</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/appnanoelectronics">Nanoelectronics</a></li> </ul> </li> </ul>
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<li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>Methods</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>DNA origami design</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methgeneraldesignstrategy">General
design strategy</a></li>
<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methmodifications%20">Modifications</a></li> </ul>
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<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methplasmidconst">Plasmid
construction</a></li>
<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methprotprodandisol">Protein
production and isolation</a></li>
<li class="pureCssMenui"><a class="pureCssMenui" href="#"><span>Protein characterization</span></a><!--[if lte IE 6]><table><tr><td><![endif]--> <ul class="pureCssMenum"> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methemsa">EMSA</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methalphascreen">AlphaScreen
assay</a></li>
<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methbret">BRET
assay</a></li>
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<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methafm">AFM</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/methtimeline">Timeline</a></li> </ul>
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<li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/team">Team</a></li> <li class="pureCssMenui"><a class="pureCssMenui" href="http://openwetware.org/wiki/Biomod/2011/Slovenia/BioNanoWizards/acknowledgments">Acknowledgments</a></li>
</ul> <!-- End PureCSSMenu.com MENU --> </div> </div> </div> <!-- end #header --> <div id="page" class="container"> <div id="content"> <div class="post"> <div class="entry"><big><big><big><big><span
style="color: black; font-weight: bold;">AFM</span></big></big></big></big><br>
<br><br> <table
style="margin: 3px 0px 20px 20px; width: 444px; height: 50px; float: right;" border="0" cellpadding="0" cellspacing="0"> <tbody> <tr> <td style="text-align: center;"><img style="padding: 0pt 0pt 5px; width: 460px; height: 306px;" alt="" src="http://openwetware.org/images/4/4e/Vidafm.JPG"></td> </tr> <tr style="font-family: Arial; font-weight: bold;"> <td style="text-align: justify;">Figure 49.<span style="font-weight: normal;"> Vid operating our atomic force
microscope</span></td>
</tr> </tbody>
</table> <span style="font-family: Arial;"> We deposited the sample (typically 5 µl with ~5 nM DNA origami purified of excess staple strands) containing rectangles with DNA origami add-ons on a freshly cleaved mica (grade V1, Ted Pella), incubated for 60 s and then rinsed with 100 µl of buffer contained in the sample and then two times with 100 µl of MQ water. The sample was dried under nitrogen stream. Atomic force microscopy measurements were performed on dried samples in tapping mode on Agilent Technologies 5500 Scanning Probe Microscope using either silicon cantilevers ARROW-NCR or SSS-NCH (Nanoworld). Scan rate was generally 1 Hz and below, with engaging amplitude of 300-400 mV. </span><br
style="font-family: Arial;">
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