The concentration of both the DNA and fluorophores in the sample was calculated by measuring the absorption and using Lambert-Beers law. The calculations were done using the wavelengths 260nm for DNA, 550nm for Cy3 and 649nm for Cy5. The extinction coefficients for the DNA were calculated using the calculator on RiboTasks webpage. The sequences used can be found in the Supplementary. The extinction coefficients for the fluorophores were data from previous work. The cuvette used had a path length of 3mm. The concentration of the fluorophore are higher than that of DNA in all four samples, which can be interpreted as there being a surplus of fluorophores in each sample. It is therefore expected that all of the FRET staples to have a fluorophore attached. It is also noted that the difference in concentration indicate a lack of purification after labeling of the staples, which is not a problem because the assembled structure is purified before the FRET measurements.