Biomod/2011/Aarhus/DanishNanoArtists/Overview/Achievements

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• Team
  • Mie
  • Irene
  • Jens
  • Hans Christian
  • Steffen
  • Oompa-Loompas
  • Acknowledgements
• Idea
  • Video
  • Goals
• Project
  • Self-assembly of the structure
  • Gen knock-down using the RNAi pathway
  • Controlled opening of the structure
  • Methods                   >
    • Gels electrophoresis
    • SAXS
    • FRET
    • Dicer
    • Dual Luciferase assay
  • Abbreviations
• Overview
  • Goals
  • Achievements
  • Future work
  • Perspectives
• Supplementary
  • Protocols
  • Sequences
  • SAXS
  • FRET
  • Luciferase
  • Literature

Achievements

• We have designed and successfully assembled a 3D RNA origami structure in the form of a octahedron.

• The assembly of the octahedron was verified and characterized using band shift assays, HPLC, FRET, and SAXS.

• We have achieved opening of the RNA/DNA hybrid octahedron as a response to the presence of a specific micro RNA, as seen by FRET.

• We have succeeded in producing small interfering RNAs from the origami structure by designing the RNA octahedron with 3’ overhangs, serving as recognition sites for specific cleavage by Dicer.

• We achieved knockdown of Renilla Luciferase by transfection of the RNA octahedron with 3’ overhangs into H1299 cells.

• We have successfully produced an RNA octahedron which can both deliver and release drugs as a response to specific micro RNAs, and act as a drug itself, upon cleavage into siRNA.