Biomod/2011/Aarhus/DanishNanoArtists/Overview/Achievements

From OpenWetWare
Jump to navigationJump to search

<html> <style>

  1. column-one { display:none; width:0px;}

.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}

  1. content { width: 0px; margin: 0 auto auto 0; padding: 0em 0em 0em 0em; align: center;}
  1. column-content {width: 0px; float: left; margin: 0 0 0 0;padding: 0;}

.firstHeading {display:none; width:0px;}

  1. globalWrapper{width:848px; background-color: #ffffff; margin-left: auto; margin-right: auto}
  1. column-one {display:none; width:0px;background-color: #ffffff;}
  1. content{ margin: 0 0 0 0; align: center; padding: 12px 12px 12px 12px; width: 824px;background-color: #ffff; border: 0;}
  1. bodyContent{ width: 800px; align: center; background-color: #ffffff;}
  1. column-content{width: 824px;background-color: #ffff;}
  1. footer{position: center; width: 848px}

a:first-letter { 

 color:#f71c1a;

}

  1. nav, #nav ul{
    margin-left:auto;
    margin-right:auto;
    margin-bottom: 0;
    padding:0px;
    list-style: none;
    list-style-type:none;
    font-weight:bold;
    list-style-position:outside;
    position:relative;
    line-height:2em;
    width:706px;

}
#nav a:link, #nav a:active, #nav a:visited{
   display:block;
   padding:0px 5px;
   border:1px solid #fff;
   color:#000;
   text-decoration:none;
   background-color:#fff;
}
  1. nav a:hover{
   background-color:#000;
   color:#fff;

}

  1. nav li{
   margin: 0;
   margin-bottom: -1px;
   float:left;
   list-style: none;
   position:relative;
   width:141px;

}

  1. nav ul {
   position:absolute;
   list-style: none;
   width:141px;
   top:2em;
   display:none;

}

  1. nav li ul a{
   margin: 0;
   list-style: none;
   width:141px;
   height:auto;
   float:left;
   margin: 0;

}

  1. nav ul ul{
       list-style: none;

top:auto; }

  1. nav li ul ul {
   list-style: none;
   left:141px;
   margin:0px 0 0 10px;
   }
  1. nav li:hover ul ul, #nav li:hover ul ul ul, #nav li:hover ul ul ul ul{
   display:none;
   }
  1. nav li:hover ul, #nav li li:hover ul, #nav li li li:hover ul, #nav li li li li:hover ul{
   display:block;
   }

IMG.displogo { 

   display: block;
   margin-left: auto;
   margin-right: auto }

</style> <body> <a href="http://openwetware.org/wiki/Biomod/2011/Aarhus/DanishNanoArtists"> <IMG class="displogo" src="http://openwetware.org/images/6/67/DNALogo.png" alt=""/> </a> <script type='text/javascript' src='https://ajax.googleapis.com/ajax/libs/jquery/1.6.4/jquery.min.js'></script> <script> function mainmenu(){ $(" #nav li").hover(function(){ $(this).find('ul:first').css({visibility: "visible",display: "none"}).show(400); },function(){ $(this).find('ul:first').css({visibility: "hidden"}); }); } 

$(document).ready(function(){

mainmenu(); }); </script>

</body> </html>


Achievements

  • We have designed and successfully assembled a 3D RNA origami structure in the form of a octahedron.
  • The assembly of the octahedron was verified and characterized using band shift assays, HPLC, FRET, and SAXS.
  • We have achieved opening of the RNA/DNA hybrid octahedron as a response to the presence of a specific micro RNA, as seen by FRET.
  • We have succeeded in producing small interfering RNAs from the origami structure by designing the RNA octahedron with 3’ overhangs, serving as recognition sites for specific cleavage by Dicer.
  • We achieved knockdown of Renilla Luciferase by transfection of the RNA octahedron with 3’ overhangs into H1299 cells.
  • We have successfully produced an RNA octahedron which can both deliver and release drugs as a response to specific micro RNAs, and act as a drug itself, upon cleavage into siRNA.


<html> 

 <style>
   #images{
   }

   img.left
   {
     float: left;
   }



   img.right
   {
     float: right;
   }

 </style>
 <body>

       <a href="http://inano.au.dk/"><img class="left" src="http://openwetware.org/images/7/70/InanoLogo.jpg"/></a>
       <a href="http://au.dk/en"><img class="right" src="http://openwetware.org/images/6/68/AULogo.png"/></a>

 </body>

</html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Biomod/2011/Aarhus/DanishNanoArtists/Overview/Achievements&oldid=561137"