BME100 s2016:Group16 W1030AM L2

BME 100 Spring 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Ryanne Maxie Name: Mayar Allam Name: Tashena Jackson Name: Joseph Carrillo Name: Austin Morgan Name: Andrew Smith LAB 2 WRITE-UP Descriptive Statistics Experiment 1 We choose the t-test for the rat experiment because there were two types of groups. The groups consisted of 5 rats each with two different types of dosages. The dosages were 0mg and 10mg of lipopolysaccharide (LPS). Experiment 2 Analysis of Variance (ANOVA) which is special form of a t-test that allows you to make multiple comparisons between groups. We chose to do the ANOVA on the human tests because we had four different types of groups. The groups consisted of ten individuals which consumed four different dosages. The dosages were 0mg, 5mg,10mg,15mg of lipopolysaccharide (LPS) Results Experiment 1 "Inflammotin with rats" ‎Experiment 2 "Inflammotin with elderly human subjects" Analysis Experiment 1 Rat Test For the rats a t-test was used because there were only two groups in the study. P-Value:0.8674035 pg/mL The P-Value was greater than 0.05 causing there to be no significance between the two data sets. Experiment 2 Human Test For humans an Anova test was used since there were more than two groups in the study. P-Value:.008333333 Post-hoc Tests Summary/Discussion Based off of the analysis of the data given, the Inflammotin protein levels in the elderly humans were significantly effected depending on the dosage of lipopolysaccharide (LPS). As the dosage increased from 0mg to 15mg of LPS, the protein levels decreased. This meant that there was less inflammation in the elderly humans. We used an unpaired analysis of variance test (ANOVA) and a bonferroni correction test (t-test) to determine that the data did have significance because there were several groups being compared. Based off of the analysis of the data given, the Inflammotin protein levels in the rats had no statistical significance. We used an unpaired t-test for this trial because there were only two groups being compared. We can not conclude anything from the data provided. These different results could have been for many reasons. One of the big factors is that there were not enough groups or subjects in the rat trial to have a significant difference in the dosages. Also, the drug could've had different responses in the rats than it did in the humans due to the difference in the digestion process, immune systems, and body mass. The lowest possible dose of lipopolysaccharide that would increase Inflammotin in the elderly is 5mg. However, if a higher Inflammotin level is desired, the dosage of lipopolysaccharide would need to be increased.