BME100 s2016:Group11 W1030AM L2

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OUR TEAM Name: Stephanie Brewer Name: Xiowen Chen Name: Kyle Durrant Name: Annette Flores Name: Eric Wright Name: Brandon Taylor LAB 2 WRITE-UP Descriptive Statistics Experiment 1 Human Study Doses Average Standard Deviation Standard Error 0mg 3.834 1.523 0.481 5mg 8.932 1.593 0.504 10mg 61.62 30.11 9.521 15mg 657.9 212.9 67.33 Experiment 2 Rat Study Dose Average Standard Deviation Standard Error 0mg 10.516 2.226 0.995 10mg 11.112 7.402 3.310 Results Experiment 1 human study analysis ‎Experiment 2 rat study analysis Analysis Experiment 1 Since there are more than 2 groups, so we use one-way a ANOVA to do data analysis, the p-value is way below than academic standard 0.05, which proves there is a statistically difference among different dose of PLS on human. Then we did post-hoc test to complete t-test for each comparison, it turns out all comparisons are statistically significant. So the data derived is accurate and valid. Experiment 2 For rat data, we only have 2 groups. So we use T-test to investigate the difference between 2 groups. However, the P-value derived is ridiculously high. So the there isn't a statistically difference among the different dose of PLS on rat. Summary/Discussion We did two experiments in order to investigate the effect of LPS to increase inflammotin on human. And one of the two we choose rat as object for a preclinical test. We only chose two groups of rats who are injected 0 mg and 10 respectively. according to the acquired data, the average of two groups' inflammation level are very close, but the 10mg group' SD is very high, after t-test, we found out that there isn't a statistically difference of the effect of different dose of LPS on rat. For the experiment on human, we had 4 groups given 0,5,10,15 mg LPS separately. The inflammotin level increased greatly with increased dose of LPS, and corresponding SD of each group also increase greatly. Through one-way ANOVA, we got very low p-value which prove the result is accurate and stable and that LPS can increase the inflammotin level in human body. Finally, we think the failure of LPS on rats and success on human may give might be contributed to the large number of objects and groups on human experiment, or it's because the difference between human and rat themselves that lead to opposite experiment results.