BME100 s2016:Group11 W1030AM L2

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Contents

OUR TEAM

Name: Stephanie Brewer
Name: Stephanie Brewer
Name: Xiowen Chen
Name: Xiowen Chen
Name: Kyle Durrant
Name: Kyle Durrant
Name: Annette Flores
Name: Annette Flores
Name: Eric Wright
Name: Eric Wright
Name: Brandon Taylor
Name: Brandon Taylor

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1
Human Study

Doses Average Standard Deviation Standard Error
0mg 3.834 1.523 0.481
5mg 8.932 1.593 0.504
10mg 61.62 30.11 9.521
15mg 657.9 212.9 67.33


Experiment 2
Rat Study

Dose Average Standard Deviation Standard Error
0mg 10.516 2.226 0.995
10mg 11.112 7.402 3.310




Results

Experiment 1
human study analysis Image:anovatest.jpg

Image: human analysis.jpg


Experiment 2
rat study analysis Image:t.test.jpg




Analysis

Experiment 1
Since there are more than 2 groups, so we use one-way a ANOVA to do data analysis, the p-value is way below than academic standard 0.05, which proves there is a statistically difference among different dose of PLS on human. Then we did post-hoc test to complete t-test for each comparison, it turns out all comparisons are statistically significant. So the data derived is accurate and valid.


Experiment 2
For rat data, we only have 2 groups. So we use T-test to investigate the difference between 2 groups. However, the P-value derived is ridiculously high. So the there isn't a statistically difference among the different dose of PLS on rat.




Summary/Discussion

We did two experiments in order to investigate the effect of LPS to increase inflammotin on human. And one of the two we choose rat as object for a preclinical test. We only chose two groups of rats who are injected 0 mg and 10 respectively. according to the acquired data, the average of two groups' inflammation level are very close, but the 10mg group' SD is very high, after t-test, we found out that there isn't a statistically difference of the effect of different dose of LPS on rat. For the experiment on human, we had 4 groups given 0,5,10,15 mg LPS separately. The inflammotin level increased greatly with increased dose of LPS, and corresponding SD of each group also increase greatly. Through one-way ANOVA, we got very low p-value which prove the result is accurate and stable and that LPS can increase the inflammotin level in human body. Finally, we think the failure of LPS on rats and success on human may give might be contributed to the large number of objects and groups on human experiment, or it's because the difference between human and rat themselves that lead to opposite experiment results.

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