BME100 s2015:Group7 9amL5
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LAB 5 WRITE-UP
Smart Phone Camera Settings
The cellular device used in the data collecting process requires modifications to the setup. Since the Android device for this collection process does not properly fit the cradle provided, the group balances the Android phone on its bottom and--with the help of a brace--prop up against the elevated fluorimeter. With this setup, it eliminates the issue of stability, is allows easier adjustment if desired , and is easier to deconstruct upon clean-up.
Final DNA concentration in SYBR Green I solution (The Means Of Each Sample Taken)
Representative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
PCR Results Summary
SNP Information & Primer Design
Background: About the Disease SNP SNP is the most common genetic variation. In fact, on average most SNP have little or no negative effect for the human population. Instead SNP is used as a tool for discovery. Importantly, SNP is used by scientists to mark and find the associated diseases. There is much still to discover about SNP. The more we learn, the more beneficial SNP seems to become.
Primer Design and Testing
Using the method of specific primer design can help identify certain portion of A DNA sample after its PCR cycle. Specific primer can be cataloged into databases, with appropriate characteristics associated to a specific sequences. In this case, a reliable online database assists in matching the disease-associated allele found within a series of nucleotides (rs268). From here, the PCR cycle results in the multiplication of this desire portion; assisting in furthering the research of the DNA strand. And since SNP marks the varies degree of differential within a DNA strand, it serves to assist in the crucial discovery of other important differences.