User:Samiye Yaman/Notebook/Lab 571/2012/02/21: Difference between revisions

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==Description==
==Description==
'''Part A'''
'''Part A'''
Syntsizing 2 solutions of AuNP with different ratio:


---Solution 1 (Au/BSA mole ratio = 70)
Syntsizing purple fibers (Au/BSA mole ratio = 166)


*Combine 1 mL of 3.66 mM HAuCl4 and 3.02 mL of 0.0173 mM BSA in a test tube.
*Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.


*Dilute to 10 mL with water.
*Dilute to 10 mL with water.


*Place in oven.(80°C)
*Place in oven. (75°C)


---Solution 2 (Au/BSA mole ratio = 166)
*Leave the test tube in oven for 3 hrs.
 
'''Part B'''


*Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
Determining the effect of tris buffer on 166 Au/BSA ratio solution


*Dilute to 10 mL with water.
*Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 (week 1). [[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]]


*Place in oven. (80°C)
*Centrifuge for 10 min at 13,200 rpm.


'''Part B'''
*Take UV-vis data of supernatant.
Leave solutions in oven for 2.5 hr.

Revision as of 13:04, 29 February 2012

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Description

Part A

Syntsizing purple fibers (Au/BSA mole ratio = 166)

  • Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
  • Dilute to 10 mL with water.
  • Place in oven. (75°C)
  • Leave the test tube in oven for 3 hrs.

Part B

Determining the effect of tris buffer on 166 Au/BSA ratio solution

  • Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 (week 1). [[1]]
  • Centrifuge for 10 min at 13,200 rpm.
  • Take UV-vis data of supernatant.