User:Samiye Yaman/Notebook/Lab 571/2012/02/15

Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Objective

-Synthesizing AuNP solutions. (purple solution)

-UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012

[[1]]

-Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012

[[2]]

Description

Synthesizing AuNP

1. Combine 2 mL of 3.66 mM HAuCl₄ and 6.04 mL of 0.0173 mM BSA in a test tube.
2. Dilute to 20 mL with water.
3. Place in oven at 80°C.
4. Leave solutions in oven for 3 hr.
5. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time.

Determining the effect of tris buffer on 166 Au/BSA ratio solution

1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12.
2. Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
3. Allow fibers to settle.
4. Take UV-vis data of tris/fiber solution.

Data