User:Samiye Yaman/Notebook/Lab 571/2012/02/21

Biomaterials Design Lab

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers Description Part A Syntsizing purple fibers (Au/BSA mole ratio = 166) Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube. Dilute to 10 mL with water. Place in oven. (75°C) Leave the test tube in oven for 3 hrs. Part B Determining the effect of tris buffer on 166 Au/BSA ratio solution Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 [[1]] and modified on [[2]] Centrifuge for 10 min at 13,200 rpm. Take UV-vis data of supernatant. Data