User:Samiye Yaman/Notebook/Lab 571/2012/02/15: Difference between revisions

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== '''Statement of Work''' ==
== '''Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers''' ==




The proposed project will address the following four topics:
==Objective==


1. Determine the process by which refolding buffers affect the nanoparticle aggregate using UV/VIS spectroscopy.  
-Synthesizing AuNP solutions. (purple solution)


2. Test methods of removing gold nanoparticles from BSA protein while keeping them in solution.
-UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012


3. Determine optimum methods of adding nanoparticle solution to refolding buffer.
[[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]]


4. Compare various refolding buffer options to determine the most stable solution using UV/VIS spectroscopy.
-Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012
 
[[http://openwetware.org/wiki/User:Samiye_Yaman/Notebook/Lab_571/2012/01/31]]
 
 
==Description==
 
<b>Synthesizing AuNP</b>
 
# Combine 2 mL of 3.66 mM HAuCl<sub>4</sub> and 6.04 mL of 0.0173 mM BSA in a test tube.
# Dilute to 20 mL with water.
# Place in oven at 80°C.
# Leave solutions in oven for 3 hr.
# Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time.  
 
 
<b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b>
 
# Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]].
# Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
# Allow fibers to settle.
# Take UV-vis data of tris/fiber solution.

Revision as of 12:54, 29 February 2012

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Determining Processes of Isolating Gold Nanoparticles Using Refolding Buffers

Objective

-Synthesizing AuNP solutions. (purple solution)

-UV-Vis of Solution 1 (Au/BSA mole ratio = 70) synthesized on 31/01/2012

[[1]]

-Determining the effect of tris on solution 2 (Au/BSA mole ratio = 166) synthesized on 31/01/2012

[[2]]


Description

Synthesizing AuNP

  1. Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 20 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3 hr.
  5. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on 01/31/12 over time.


Determining the effect of tris buffer on 166 Au/BSA ratio solution

  1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12.
  2. Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution).
  3. Allow fibers to settle.
  4. Take UV-vis data of tris/fiber solution.
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