User:Mike Barnkob: Difference between revisions

Revision as of 03:52, 18 December 2014

Coloning

CRISPR / Cas9
- Designing sgRNA oligos
- Cloning oligos into backbone
- Transforming bacteria with construct
- Screening for inserts
Lentiviral work
- Lentiviral production
- Infecting target cells

Bacterial work

Immunology

Tissue culture work

Mouse work

Visualization

I work on:

Past:

[[Mike_Barnkob:Projects/

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 *Mike Barnkob
 *Weatherall Institute for Molecular Medicine, University of Oxford
-*Email: [[Special:Emailuser/Mike Barnkob|Say hallo]].
 *Website: [http://www.mikebarnkob.dk www.mikebarnkob.dk].
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 Coloning
-* Gibson assembly
+* CRISPR / Cas9
-** Test
+** Designing sgRNA oligos
-*
+** Cloning oligos into backbone
-* CRISPR/Cas9 guide sequences
+** Transforming bacteria with construct
+** Screening for inserts
+* Lentiviral work
+** Lentiviral production
+** Infecting target cells
+Bacterial work
+* Making LB agar plates
+*
 Immunology
@@ Line 20: / Line 26: @@
 Tissue culture work
+* Medium
+** R10
+** D10
+** DMEM for 293TLA cells
+* Thawing cells
+* Freezing down cells
+* Split cells
+* Count cells
+* Harvest splenocytes
+* Harvest BMDCs
+* ''In vitro'' expansion of T cells
+* Soft agar assay
 Mouse work
+* Bleeding
+* Harvesting bone-marrow DC's
+Visualization
+* Long-time co-culture and visualization with live-dead marker
 ==Projects==
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 '''Past:'''
-[[Mike_Barnkob:Projects/Liquid handling robot|Liquid handling robot]]. An attempt to create a small liquid handling robot to do simple experiments with.
+[[Mike_Barnkob:Projects/
-[http://2009.igem.org/Team:SDU-Denmark Bacto Bandage]. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).