User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions
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==12/18/12== | ==12/18/12== | ||
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* | * TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C | ||
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''' | '''TRIzol-RNeasy prep'''<br> | ||
# "K562 1": 1:2 dilution cells, +DNA | # "K562 1": 1:2 dilution cells, +DNA | ||
# "K562 1": 1:2 dilution cells, no DNA | # "K562 1": 1:2 dilution cells, no DNA | ||
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# "K562 1": 1:8 dilution cells, no DNA | # "K562 1": 1:8 dilution cells, no DNA | ||
* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture | * Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (did this 4x for each culture); discard remainder of culture | ||
* Pellet cells at r.t./ | * Pellet cells at r.t./ 5 min./ 3000 rpm | ||
* Discard supernatant | * Discard supernatant | ||
* Resuspend pellet in 500 μL | * Resuspend pellet in 500 μL TRIzol | ||
* Store at -80°C for next steps (Carly) | * Store at -80°C for next steps (Carly) | ||
Revision as of 20:23, 4 June 2014
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12/18/12
TRIzol-RNeasy prep
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