User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/26

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12/26/12

  • ChIP-seq enrichment: PcTF vs. PcTF mock for select genes

ChIP: PcTF vs. PcTF mock

  • Completed PcTF (lane 1) and PcTF mock (lane 2) BAM --> assembly workflow
  • Open in SeqMan pro to see raw values for four genes from 2011 paper.


Workflow

  • Open SeqMan Pro. File > Open > ...navigate to the desired ###.assembly file in the SeqMan NGen Projects folder. Processing takes 5 -10 minutes.
  • In an internet browser, go to the UCSC genome browser. Select Feb. 2009 GRCh37/hg19.
  • Enter a gene name. Click [Submit].
  • Take note of the chromosome number.
  • Find the appropriate assembly template in the SeqMan Pro (e.g., NC_000001 for chromosome 1). Double click it.
  • Select Contig > Strategy View to view the enrichment histogram.
  • Select Features > Show Feature Table. Find the gene name. Click on it and the strategy view window will jump to that gene.
  • Click the gene symbol. Zoom in. Take a snapshot


From the Haynes & Silver JBC 2011 paper, ChIP-seq experiment

1. Name: CDKN2A, chr9

  • SeqMan Pro template NC000009(1>141215022) NC_000009

2. Name: MMP12, chr 11

  • SeqMan Pro template NC000011(1>135008327) NC_000011

3. Name: NPPA, chr 1

  • SeqMan Pro template NC000001(1>249240538) NC_000001

4. Name: EOMES, chr 3

  • SeqMan Pro template NC000003(1>197960493) NC_000003




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