12/18/12
- TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C
TRIzol-RNeasy prep
- "K562 1": 1:2 dilution cells, +DNA
- "K562 1": 1:2 dilution cells, no DNA
- "K562 1": 1:4 dilution cells, +DNA
- "K562 1": 1:4 dilution cells, no DNA
- "K562 1": 1:8 dilution cells, +DNA
- "K562 1": 1:8 dilution cells, no DNA
- Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (did this 4x for each culture); discard remainder of culture
- Pellet cells at r.t./ 5 min./ 3000 rpm
- Discard supernatant
- Resuspend pellet in 500 μL TRIzol
- Store at -80°C for next steps (Carly)
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