User:Karlena L. Brown/Notebook/PVOH Research/2013/03/27: Difference between revisions
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==OBJECTIVES== | ==OBJECTIVES== | ||
* Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13 | * Remove all excess oil from microsphere samples prepared on '''03/20/13''' and '''03/22/13''' | ||
* Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil) | * Clean and dry microsphere samples with hexanes prepared '''03/20/13''' and '''03/22/13''' (dissolving excess mineral oil) | ||
==Microspheres: Separation & Vacuum Filtration Procedures== | |||
==Microspheres: Separation & Vacuum Filtration Procedures 2== | |||
'''RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13''' | '''RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13''' | ||
For the Protocol originally discussed on 03/08/13, some modifications that occurred included: | |||
For the Protocol originally discussed on '''03/08/13''', some modifications that occurred included: | |||
* A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes | * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes | ||
* Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min | * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min | ||
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==Notes== | ==Notes== | ||
* On | * On '''03/29/13''' a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used. | ||
* On | * On '''03/29/13''' a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used. | ||
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Revision as of 19:07, 8 April 2013
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OBJECTIVES
Microspheres: Separation & Vacuum Filtration Procedures 2RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13 For the Protocol originally discussed on 03/08/13, some modifications that occurred included: * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry Notes
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