OBJECTIVES
- Prepare controlled microsphere samples – PVOH 130K and 146K
- Finish decanting microsphere samples prepared 03/22/13
- Dry microsphere samples rinsed with hexanes (03/27/13) in preparation for DSC and X-ray
- Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing
Mixed PVOH Microsphere Samples & Dye Preparations
RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
| PVOH Type
|
PVOH Mass (g)
|
H2O Added (mL)
|
Safflower Oil Added (mL)
|
| 130K |
0.10400 |
20 |
35
|
| 140K |
0.1022 |
20 |
35
|
Notes
- Samples previously rinsed with hexanes 03/27/13 and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
- Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
- The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
- For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.
| 
PVOH Research
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