User:Karlena L. Brown/Notebook/PVOH Research/2013/03/27

PVOH Research

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OBJECTIVES Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13 Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil) Microspheres: Separation & Vacuum Filtration Procedures 2 RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13 For the Protocol originally discussed on 03/08/13, some modifications that occurred included: * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry Notes On 03/29/13 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used. On 03/29/13 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.