User:Karlena L. Brown/Notebook/PVOH Research/2013/03/27: Difference between revisions

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(Autocreate 2013/03/27 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
 
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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
* Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil)
'''(Actual Mass = 0.4934g)'''
==Microspheres: Separation & Vacuum Filtration Procedures==
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
'''RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13'''
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
For the Protocol originally discussed on 03/08/13, some modifications that occurred included:
'''(Actual Mass = 0.9965g)'''
      * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
      * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min
 
      * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry
'''Standard PVOH Film Protocol:'''
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
 
'''Notes:'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
* Heat was reduced when solutions began to boil too rapidly
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)


==Notes==
* On 2013/03/29 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used. 
* On 2013/03/29 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.


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Revision as of 18:16, 8 April 2013

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OBJECTIVES

  • Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13
  • Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil)

Microspheres: Separation & Vacuum Filtration Procedures

RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13 For the Protocol originally discussed on 03/08/13, some modifications that occurred included: 

     * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes
     * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min
     * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry

Notes

  • On 2013/03/29 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used.
  • On 2013/03/29 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.


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