User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/29

From OpenWetWare
Jump to navigationJump to search
iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Grow in solid medium BBa_B0015 and liquid for extraction.
  • See gel from extractions of pUX19 cut with SmaI.
  • Digestion of pSB1AK3/BBa_B0015 with XbaI to see size.

Gel of SmaI restriction

I expect 6.2Kbp~

Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.

Extraction

Extraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep.

I just need it to see concentration for digestion.

Digestion

Reactives μL
XbaI 1
Buffer4 NEB10X 2
BSA10X 2
DNA of pSB1AK3/BBa_B0015 12
H2O 3
Total 20

Over night reaction.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Jose_Fabricio_Lopez/Notebook/Logbook/2011/07/29&oldid=527259"