User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28

iGEM 2011

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Objectives Plasmid extraction of pUX19XKR5, for PCR of Inc-RepC. Transformation of terminator BBa_B0015 for plasmid standardization. Restriction of today´s plasmid extraction. Transformation Miguel's lab method. Plasmid extraction Miguel's lab miniprep. Restriction Restriction performed with SmaI, unique site in plasmid for Extraction 1 ,Extraction 2 and Today´s extraction. Extraction 1 Extraction 2 Today´s Buffer4 2μL 2μL 2μL SmaI 1μL 1μL 1μL DNA 10μL 10μL 10μL H2O 7μL 7μL 7μL Total 20μL 20μL 20μL I expect 1 band in cut DNA, I will choose for PCR the right plasmid and cleanest.