User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/29: Difference between revisions

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=Objectives=
=Objectives=
* Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015].
* Grow in solid medium [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] and liquid for extraction.
* See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| extractions]] of pUX19 cut with SmaI.
* See gel from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28| extractions]] of pUX19 cut with SmaI.


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Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.
Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.
==Extraction==
Extraction of plasmid with biobrick BBa_B0015, performed with miniprep.
[[Image:PartBBa B0015.KAres.29jul2011.JPG|120px]]


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__NOTOC__
__NOTOC__

Revision as of 10:57, 30 July 2011

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Objectives

  • Grow in solid medium BBa_B0015 and liquid for extraction.
  • See gel from extractions of pUX19 cut with SmaI.


Gel of SmaI restriction

I expect 6.2Kbp~

Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.

Extraction

Extraction of plasmid with biobrick BBa_B0015, performed with miniprep.