User:Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/03/14: Difference between revisions
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==new CK2== | |||
* Induction will be done at 2pm | |||
* Take 2 500ul total induced samples, spin, wash, spin, resuspend | |||
* Spin 10min 6K 4C in large Oakridge bottle, discard LB | |||
* Wash in 25ml MK buffer, transfer to small Oakridge tube | |||
* Spin 10min 6K 4C, discard MK buffer | |||
* Resuspend in 30ml MK buffer + 300ul 100x protease inhibitor | |||
* Store 5ml in a conical in -80C | |||
* Store 25ml in a conical in -80C | |||
==Substrates== | |||
* Bad news: did PCR cleanups wrong, yielded very low concentrations | |||
* Good news: calculations prove that there is enough PCR product to do T4 treatment for cloning | |||
Revision as of 10:12, 14 March 2014
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