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Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/03/13
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Notebook
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Identifying Kinase Substrates of PfCK2
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2014
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03
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new CK2
2 colonies of RIPL were chosen and grown ON in 5ml LB and 5ul Amp
Inoculate 2.5ml into 250ml
Grow to OD600 = 0.5 at 37C
Cool to 20C for 30mins
Add 25ul to achieve 0.1mM IPTG induction
Incubate 20hrs at 20C
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