User:Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/03/14

Project name

Main project page Previous entry      Next entry

new CK2 Induction will be done at 2pm Take 2 500ul total induced samples, spin, wash, spin, resuspend Spin 10min 6K 4C in large Oakridge bottle, discard LB Wash in 25ml MK buffer, transfer to small Oakridge tube Spin 10min 6K 4C, discard MK buffer Resuspend in 30ml MK buffer + 300ul 100x protease inhibitor Store 5ml in a conical in -80C Store 25ml in a conical in -80C Substrates Bad news: did PCR cleanups wrong, yielded very low concentrations Good news: calculations prove that there is enough PCR product to do T4 treatment for cloning Bad news: the pET-30 kit has not come in yet