User:Benjamin Friedel/Notebook/CHEM 471/2016/03/01

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Objectives

  1. Work on Disc Diffusion Antibacterial Procedure and Prepare Reagents
  2. UV Vis on 02/24/16 Nanoparticle Reactions

Materials

  1. Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
    1. Integration time= 10ms and Average =100.
    2. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.

Procedure

Materials for Agar Disc Diffusion

  1. Mueller Hinton Agar

-Dissolved Agar packet in 1L of water over hot plate -Autoclaved on wet cycle

    1. Whatman Membrane Filter Paper

-autoclaved in dry cycle

      1. Protein-Nanoparticle samples and Ampicillin Control Dilutions

AMP Table

Concentration (mg/L) Concentration (ug/uL) Volume (ul) Mass (ug)
4 0.004 25 0.1
8 0.008 25 0.2
16 0.016 25 0.4
32 0.032 25 0.8
64 0.064 25 1.6


Example Nanoparticle Sample Dilutions

Concentration (x)
1
0.5
0.25
0.125
0.0625


  1. Reaction mix data can be found at the link SRB Lab Notebook Data

Due to time constraints, the actual diffusion assay was not performed this day



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