Objectives
- Set up 20ml AgNP and AuNP reactions for Casein, Soy, Whey, Rice, and Pea
- Run UV Vis for 02/24/16 reaction
Materials
- Used same nutritional proteins on 02/02/16
- Protein and gold stocks prepared fresh every day
- Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
- Integration time= 10ms and Average =100.
- A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.
Procedure
- Prepare 20ml reaction to synthesize large quantities of reactions known to work (see link below for specifics on reaction mix)
- Note that spectra of reaction mixes before reacting were taken
- Reaction mix and measurement data can be found at the link SRB Lab Notebook Data
and the reaction itself was run on 02/24/16, the reaction mix planning and UV Vis from 02/17/16 was performed on the 02/23/16
Results
- Color change notes and UV-VIs information can be found at the link SRB Lab Notebook Data
- Whey and Soy were in neutral reaction, Pea, Casein and Rice protein were basic - all experience color change (silver=yellow, red/purple = gold)
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