User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/06

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(Autocreate 2013/08/06 Entry for User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli)
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'''List title'''
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'''[http://openwetware.org/wiki/User:Behzad_Damadzadeh/Notebook/PcTF_Subcloning_in_E-coli/2013/08/05 Miniprep Transformed Plasmids]'''
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# Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
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# Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
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# Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo
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'''Miniprep result'''
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{| {{table}}
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|-
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| Part number || 260 || 280 || 260/280 || ng/ul
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|-
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| F2620 miniprep 1 || 0.018 || 0.012 || 1.535 || 18.413
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|-
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| F2620 miniprep 2 || 0.032 || 0.019 || 1.663 || 32.028
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|-
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| J06702 miniprep 1 || 0.009 || 0.006 || 1.367 || 8.633
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|-
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| J06702 miniprep 2 || 0.035 || 0.021 || 1.696 || 35.007
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|}

Revision as of 16:57, 8 August 2013

PcTF Subcloning in E. coli Main project page
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08/06/2013

Miniprep Transformed Plasmids

  1. Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
  1. Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
  1. Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo

Miniprep result

Part number 260 280 260/280 ng/ul
F2620 miniprep 1 0.018 0.012 1.535 18.413
F2620 miniprep 2 0.032 0.019 1.663 32.028
J06702 miniprep 1 0.009 0.006 1.367 8.633
J06702 miniprep 2 0.035 0.021 1.696 35.007
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