User:Behzad Damadzadeh/Notebook/PcTF Subcloning in E-coli/2013/08/05

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08/06/2013

Transformation

Transformation of Gal4-VP64 parts in BL21-DE3for transactivation of Cyan flourescent reporter gene.

  • Parts
BioBrick Part Vector Description
KAH59 PcVN [K : Gal4 DB : mCh : mCh] + VP64-NLS
KAH60 PcVN [K : Gal4 DB : mCh] + VP64-NLS
KAH54 PcVN [[K : Gal4 DB] + VP64-NLS
KAH59 PcN (MV5) [K : Gal4 DB : mCh : mCh] + NLS
KAH66 PcN (MV5) [K : Gal4 DB : mCh : mCh : VP64] + NLS
KAH36 V0120 [K : mCh : mCh] in pcDNA3.1+/VP64-NLS vector
  • 1μL of plasmid and 9μL dH2O.
  • 40μL of BL21-DE3 competent cells.
  • Culture on AMP agar plates in 37°C incubator overnight.
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