08/06/2013
Miniprep Transformed Plasmids
- Miniprep the transformed plasmids (GAL4-VP64) to test the transfection.
- Pick two colonies of each plate and grow in 2mL of LB broth with 100μg/mL AMP for 10 hrs.
- Miniprep the plasmids with Zyppy Plasmid Miniprep Kit from Zymo
Miniprep result
Part number |
260/280 |
ng/ul |
260/280 |
ng/ul
|
KAH59 (MV5) |
140.198 |
1.857 |
277.719 |
1.832
|
KAH54 (PCVN) |
169.122 |
1.824 |
217.267 |
1.832
|
KAH59 (PCVN) |
203.300 |
1.773 |
66.2 |
1.829
|
KAH60 (PCVN) |
33.877 |
1.988 |
169.931 |
1.71
|
KAH66 (MV5) |
121.409 |
1.814 |
174.819 |
1.822
|
KAH36 |
162.434 |
1.832 |
130.043 |
1.832
|
- KAH59 (MV5) produce red pellet after transformed E-coli centrifugation.
|