User:Beatriz Gimenez De C./Notebook/571/2014/09/23: Difference between revisions

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==Had to leave because was indisposed==
==tasks==
*Retreive electrophoresis samples prepared and refrigerated las week
** Preparations : 50 μL of 1g/L Lysozyme stock solution and dilute to 5 mL with 50 mM Glycine stock solution in volumetric flask.
*** Take 10 μL of diluted solution (Lysozyme stock and glycine solution) and 10 μL of running buffer (prepared by Dr. Fox) and add to 1.5 mL centrifuge tube, vortex for 5-10 seconds.
*** 50 μL of 30:1 [Au]:[Lysozyme] colloid solution, dilute + take 10μL of diluted solution and 10μL of running buffer solution and add to 1.5 mL centrifuge tube + vortex for 5-10 seconds
*** 50 μL of 30:1 [Au]:[BSA] colloid solution, dilute + take 10 μL of diluted solution and 10 μL running buffer and add to centrifuge tube. + vortex for 5-10 seconds
*** 50 μL of 1g/L BSA stock solution (prepared by Dr. Fox), dilute + take 10 μL of diluted solution + 10 μL of running buffer + add to 1.5 mL centrifuge tube + vortex for 5-10 seconds


* See Alicia's or Khyra's notebook
**Load 15 μL of samples (stated above, after 1 week refrigerated storage)
*** Well 3: Lysozyme
*** Well 5: BSA Colloid
*** Well 7: Lysozyme Colloid
*** Well 9: Soy
*** Well 11: All Blue Protein Standard (provided by Bio-Rad)
 
** Run Electrophoresis for 30 minutes at 200 V
** Staining Gel process (repeated twice) -> Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) during 30 minutes + Place gel in Stain Solution duringr 1 hour + Place gel in Destain Solution during 15 minutes
 
==note==
* had to leave early because indisposed Alicia's or Khyra's notebook for further detail




Revision as of 11:41, 17 December 2014

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tasks

  • Retreive electrophoresis samples prepared and refrigerated las week
    • Preparations : 50 μL of 1g/L Lysozyme stock solution and dilute to 5 mL with 50 mM Glycine stock solution in volumetric flask.
      • Take 10 μL of diluted solution (Lysozyme stock and glycine solution) and 10 μL of running buffer (prepared by Dr. Fox) and add to 1.5 mL centrifuge tube, vortex for 5-10 seconds.
      • 50 μL of 30:1 [Au]:[Lysozyme] colloid solution, dilute + take 10μL of diluted solution and 10μL of running buffer solution and add to 1.5 mL centrifuge tube + vortex for 5-10 seconds
      • 50 μL of 30:1 [Au]:[BSA] colloid solution, dilute + take 10 μL of diluted solution and 10 μL running buffer and add to centrifuge tube. + vortex for 5-10 seconds
      • 50 μL of 1g/L BSA stock solution (prepared by Dr. Fox), dilute + take 10 μL of diluted solution + 10 μL of running buffer + add to 1.5 mL centrifuge tube + vortex for 5-10 seconds
    • Load 15 μL of samples (stated above, after 1 week refrigerated storage)
      • Well 3: Lysozyme
      • Well 5: BSA Colloid
      • Well 7: Lysozyme Colloid
      • Well 9: Soy
      • Well 11: All Blue Protein Standard (provided by Bio-Rad)
    • Run Electrophoresis for 30 minutes at 200 V
    • Staining Gel process (repeated twice) -> Place gel in Fixative Solution (40% methanol, 10% acetic acid, 50% water) during 30 minutes + Place gel in Stain Solution duringr 1 hour + Place gel in Destain Solution during 15 minutes

note

  • had to leave early because indisposed Alicia's or Khyra's notebook for further detail



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