Smolke:Protocols/Heat Shock Transformation
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Revision as of 17:29, 15 July 2012 by
Stephanie Galanie
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For each sample
1 tube of thawed chemically competent cells (keep covered in ice at all times)
1-10 uL ligation product
250 uL SOC
1 LB plate with appropriate antibiotics
Procedure
Add DNA to cells
Incubate on ice for 0-20 minutes
Heat shock at 42 °C for 30 seconds
Incubate on ice for 2 minutes
Add SOC
Incubate on rotator in 37 °C incubator for 0-60 minutes
Plate 50-250 uL
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