Smolke:Protocols/Heat Shock Transformation

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For each sample 1 tube of thawed chemically competent cells (keep covered in ice at all times) 1-10uL ligation product 1 mL SOC (optional) 1 LB plate with appropriate antibiotics Procedure Add DNA to cells Incubate on ice for 0-20 minutes Heat shock at 42C for 30 seconds Add SOC (optional) Incubate on rotator in 37C incubator for 0-60 minutes Add to plate