Smolke:Enzyme inventory: Difference between revisions
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== | ==Rules== | ||
*Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common. | |||
*Only common enzymes may have backup, which are stored in main -20C. | |||
*Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | |||
*The enzymes not listed are in the non-common enzyme cooler. | |||
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | |||
*When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard. | |||
*Backup ligases and CIP are also in the backup cooler in main -20C. | |||
==Restriction Enzymes in Stocks== | |||
{| border="1" class="wikitable" style="text-align:center" | {| border="1" class="wikitable" style="text-align:center" | ||
! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ||
|- | |- | ||
! AgeI || ||Y|| ||Y | ! AflII ||Y|| || || | ||
|- | |||
! AgeI ||Y||Y|| ||Y | |||
|- | |||
! AleI ||Y|| ||Y|| | |||
|- | |||
! ApaI ||Y|| ||YY|| | |||
|- | |||
! AscI ||Y|| || || | |||
|- | |||
! AsiSI ||Y|| ||Y|| | |||
|- | |||
! AvrII ||Y|| ||Y|| | |||
|- | |||
! BamHI ||Y||Y|| ||Y | |||
|- | |||
! BglII ||Y|| || || | |||
|- | |||
! BsaI || ||Y|| || | |||
|- | |- | ||
! | ! BsrGI ||Y|| || || | ||
|- | |- | ||
! | ! BstBI ||Y|| || || | ||
|- | |- | ||
! | ! BstXI ||Y|| || || | ||
|- | |||
! Bsu36I ||Y|| || || | |||
|- | |||
! ClaI ||Y|| ||Y|| | |||
|- | |- | ||
! DpnI ||Y|| ||Y|| | ! DpnI ||Y|| ||Y|| | ||
|- | |- | ||
! EcoRI || | ! EcoRI || ||Y|| ||Y | ||
|- | |- | ||
! EcoRV || ||Y|| ||Y | ! EcoRV || ||Y|| ||Y | ||
|- | |- | ||
! HindIII ||Y|| ||Y|| | ! FseI ||Jay's -80|| || || | ||
|- | |||
! HindIII ||Y||Y||Y|| | |||
|- | |||
! KasI || || || ||Y | |||
|- | |||
! KpnI ||Y||Y|| ||Y | |||
|- | |||
! MfeI || ||Y|| ||Y | |||
|- | |||
! NdeI ||Y|| || || | |||
|- | |- | ||
! | ! NheI ||Y||Y|| || | ||
|- | |- | ||
! NotI ||Y||Y|| ||Y | ! NotI ||Y||Y|| ||Y | ||
|- | |||
! NruI ||Y|| || || | |||
|- | |||
! PacI ||Y|| || || | |||
|- | |||
! PmeI ||Y|| || || | |||
|- | |||
! PstI ||Y|| || || | |||
|- | |||
! PvuII || || Y || || | |||
|- | |- | ||
! SacI || ||Y|| ||Y | ! SacI || ||Y|| ||Y | ||
|- | |- | ||
! SacII || Y || ||Y|| | ! SacII ||Y|| ||Y|| | ||
|- | |||
! SalI ||Y||Y|| ||Y | |||
|- | |||
! SapI ||Y|| || || | |||
|- | |- | ||
! | ! SbfI || ||Y|| || | ||
|- | |||
! SpeI ||Y|| ||Y|| | |||
|- | |||
! SphI || ||Y|| || | |||
|- | |||
! SwaI ||Y|| || || | |||
|- | |- | ||
! XbaI ||Y|| ||Y|| | ! XbaI ||Y|| ||Y|| | ||
|- | |- | ||
! XhoI ||Y|| ||Y|| | ! XhoI ||Y|| ||Y|| | ||
|- | |||
! XmaI ||Y|| || || | |||
|- | |- | ||
|} | |} | ||
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==Contact== | ==Contact== | ||
*remus |
Latest revision as of 14:40, 21 August 2014
Rules
Restriction Enzymes in Stocks
Useful Links
Contact
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