Rules
- Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
- Only common enzymes may have backup, which are stored in main -20C.
- Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
- If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
- When the working stock has been used up, replace with a new tube.
- Backup ligases and CIP are also in the backup cooler in main -20C.
Restriction Enzymes in Stocks
Enzyme |
Regular |
HF |
Regular Backup |
HF Backup
|
AgeI |
|
Y |
|
Y
|
AleI |
Y |
|
Y |
|
ApaI |
Y |
|
Y |
|
AscI |
Y |
|
|
|
AsiSI |
Y |
|
|
|
BamHI |
Y |
Y |
|
Y
|
BglII |
Y |
|
|
|
BsaI |
|
Y |
|
|
BsrGI |
Y |
|
|
|
BstBI |
Y |
|
|
|
Bsu36I |
Y |
|
|
|
ClaI |
Y |
|
|
|
DpnI |
Y |
|
Y |
|
EcoRI |
|
Y |
|
Y
|
EcoRV |
|
Y |
|
Y
|
HindIII |
Y |
|
Y |
|
KpnI |
Y |
|
Y |
|
MfeI |
|
Y |
|
Y
|
NheI |
Y |
|
|
|
NotI |
Y |
Y |
|
Y
|
NruI |
Y |
|
|
|
PacI |
Y |
|
|
|
PmeI |
Y |
|
|
|
SacI |
|
Y |
|
Y
|
SacII |
Y |
|
Y |
|
SaII |
Y |
Y |
|
Y
|
SapI |
Y |
|
|
|
SbfI |
Y |
|
|
|
SpeI |
Y |
|
|
|
SphI |
Y |
|
|
|
XbaI |
Y |
|
Y |
|
XhoI |
Y |
|
Y |
|
XmaI |
Y |
|
|
|
Useful Links
Contact
Joe
|