Registry/Measurement kit/Notebook/2007-7-30: Difference between revisions
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*Should pick some promoters to drop in. | *Should pick some promoters to drop in. | ||
*R0040 + a strong promoter + a weak promoter | *R0040 + a strong promoter + a weak promoter | ||
* | *Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong) | ||
==I2055== | ==I2055== | ||
*Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR) | *Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR) | ||
Revision as of 12:32, 30 July 2007
To Do
- See about making competent DB3.1 cells
- Test the top10 stock with a + control (pUC18)
- See what we can do with BB'd E0240 & I2057
Promoter Testers
- Should pick some promoters to drop in.
- R0040 + a strong promoter + a weak promoter
- Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
I2055
- Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)
