Registry/Measurement kit/Notebook/2007-6-13: Difference between revisions
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[http://www.neb.com/nebecomm/products/productN3200.asp 2 log ladder] | [http://www.neb.com/nebecomm/products/productN3200.asp 2 log ladder] | ||
==Overnight cultures== | |||
*inoculated 5mL of LB+5uL of Amp. with 20uL of cell suspension from the colonies denoted in section 1. |
Revision as of 16:56, 13 June 2007
Current: Yesterday | Today | Tomorrow
Colony PCR
- 1-3: #1 (<bbpart>E0240</bbpart>) -- #3,size OK
- 4-6: #2 (<bbpart>R0040</bbpart>) -- #6,size OK
- 7-9: #3 (<bbpart>I13401</bbpart>) -- #9,size OK (sort of, 7&8 are slightly smaller)
- 10-12: #4 (#12 bad) (<bbpart>J04650</bbpart>) -#11, size OK
- 13: #5 (<bbpart>B0032</bbpart>) -- #13,size OK
- note: reagents in PCR TOOLS box in fridge, Taq is in Shared polymerase in the fridge near entrance
Analytical gel
- lane 1: ladder
- lanes 2-13: samples as above
- lane 14: ladder
- lanes 15-18: jason's stuff
Overnight cultures
- inoculated 5mL of LB+5uL of Amp. with 20uL of cell suspension from the colonies denoted in section 1.