Registry/Measurement kit/Notebook/2007-6-13

From OpenWetWare

Jump to: navigation, search

Registry/Measurement_kit/Notebook

Colony PCR

  • 1-3: #1 (<bbpart>E0240</bbpart>) -- #3,size OK
  • 4-6: #2 (<bbpart>R0040</bbpart>) -- #6,size OK
  • 7-9: #3 (<bbpart>I13401</bbpart>) -- #9,size OK (sort of, 7&8 are slightly smaller)
  • 10-12: #4 (#12 bad) (<bbpart>J04650</bbpart>) -#11, size OK
  • 13: #5 (<bbpart>B0032</bbpart>) -- #13,size OK


  • note: reagents in PCR TOOLS box in fridge, Taq is in Shared polymerase in the fridge near entrance

Analytical gel

  • lane 1: ladder empty
  • lanes 2-13: samples as above
  • lane 14: ladder
  • lanes 15-18: jason's stuff
Gel from 6/13/07
Gel from 6/13/07


2 log ladder

Overnight cultures

  • inoculated 5mL of LB+5µL of Amp. with 20µL of cell suspension from the colonies denoted in section 1.
Personal tools