Lidstrom:Protocols

Back home: Lidstrom

General Lab:

• Health & Safety
• Finding Chemicals
• Dishwasher
• Autoclave
• Sterile technique
• E. Coli basics
• Preparing Freezer Cell Stocks
• Pouring Media Plates

Cell Culture:

• Tube Spec
• M9 recipe
• Terrific Broth recipe
• Bioscreen Setup

DNA/RNA:

DNA

Manipulation

• Intro: Building with DNA (in progress)
• Digestion with Restriction Enzymes
• Gibson Assembly (In progress)
• Ligation (in progress)
• Overlap Extension PCR (in progress)
• Vector Compatibility

Fancy Manipulation

• Site-Directed Mutagenesis (in progress)
• λ-Red Mediated Gene Knockouts (in progress)
• Phage Lysate Prep
• P1 Phage Transduction (in progress)

Obervation

• Colony PCR
• Agarose Gel Electrophoresis
• Sequencing with GeneWiz

Misc.

• PCR (in progress)
• Loading Dye Recipe
• Purifying DNA (in progress)
• Miniprep (in progress)
• Oligo Orders (in progress)
• Diluting Primers

Protein

• SDS-PAGE Protein Gels

E. Coli

• Competent Cell Preparation (in progress)
• Chemical Transformation (in progress)
• Electroporation (in progress)

Enzyme Assays

General

• Plate Reader Setup

in vitro pathway assays (CO₂ project)

• Cell Prep (in progress)
• Crude Extract Prep (in progress)
• Sample Analysis via UPLC-MS/MS (in progress)

Metabolomics

• Measuring 13C Incorporation Into Protein - CO2 Project