Lidstrom:Protocols: Difference between revisions
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*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
*[[Lidstrom: Bioscreen|Bioscreen Setup]] | *[[Lidstrom: Bioscreen|Bioscreen Setup]] | ||
=== Methylotrophs === | === Methylotrophs === | ||
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | *[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | ||
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*[[Lidstrom:TB|Terrific Broth recipe]] | *[[Lidstrom:TB|Terrific Broth recipe]] | ||
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | *[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | ||
=== OD measurements === | |||
* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]] | |||
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]] | |||
==DNA/RNA:== | ==DNA/RNA:== | ||
===DNA=== | ===DNA=== | ||
== PCR == | ==== PCR ==== | ||
*[[Lidstrom:Colony PCR| Colony PCR]] | *[[Lidstrom:Colony PCR| Colony PCR]] | ||
*[[Lidstrom:PCR| PCR (in progress)]] | *[[Lidstrom:PCR| PCR (in progress)]] | ||
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | ||
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | ||
==== Misc ==== | |||
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | |||
== Metabolomics == | == Metabolomics == |
Revision as of 17:23, 25 March 2014
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
OD measurements
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Protein
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)