Lidstrom:Protocols

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(Obervation)
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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
==DNA/RNA:==
==DNA/RNA:==
===DNA===
===DNA===
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====Isolation ====
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*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
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*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
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*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
====Manipulation====
====Manipulation====
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
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*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
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*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
-
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
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*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
=====Fancy Manipulation=====
=====Fancy Manipulation=====
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 +
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
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 +
==== Restriction Cloning ====
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*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
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*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
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*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
====Obervation====
====Obervation====
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*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
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*[[Lidstrom:Oligo Orders | Oligo Orders (in progress)]]
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*[[Lidstrom:Oligo Orders | Oligo Orders]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
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==E. Coli==
==E. Coli==
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*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility  (In Progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
-
*[[Lidstrom:Transformation| Transformation (in progress)]]
+
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
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*[[Lidstrom:Electroporation|Electroporation]]
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*[[Lidstrom:Competent Cells|Competent Cell Lines]]
==Enzyme Assays==
==Enzyme Assays==
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
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== Metabolomics ==
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=== CO2 project ===
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*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
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** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Revision as of 09:07, 31 March 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools