Lidstrom:Protocols
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===DNA=== | ===DNA=== | ||
| + | ====Isolation ==== | ||
| + | *[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | ||
| + | *[[Lidstrom:Genomic DNA extraction|Genomic DNA extraction]] | ||
====Manipulation==== | ====Manipulation==== | ||
| Line 27: | Line 30: | ||
*[[Lidstrom:Ligation |Ligation (in progress)]] | *[[Lidstrom:Ligation |Ligation (in progress)]] | ||
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
| + | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] | ||
=====Fancy Manipulation===== | =====Fancy Manipulation===== | ||
| Line 44: | Line 48: | ||
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | *[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | ||
*[[Lidstrom:Miniprep |Miniprep (in progress)]] | *[[Lidstrom:Miniprep |Miniprep (in progress)]] | ||
| - | *[[Lidstrom:Oligo Orders | Oligo Orders | + | *[[Lidstrom:Oligo Orders | Oligo Orders]] |
*[[Lidstrom:Diluting Primers | Diluting Primers]] | *[[Lidstrom:Diluting Primers | Diluting Primers]] | ||
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==E. Coli== | ==E. Coli== | ||
| + | *[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility (In Progress)]] | ||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
| - | *[[Lidstrom:Transformation| Transformation | + | *[[Lidstrom:Chemical Transformation|Chemical Transformation]] |
| + | *[[Lidstrom:Electroporation|Electroporation]] | ||
| + | *[[Lidstrom:Competent Cells|Competent Cell Lines]] | ||
==Enzyme Assays== | ==Enzyme Assays== | ||
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | ||
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | ||
| + | |||
| + | == Metabolomics == | ||
| + | === CO2 project === | ||
| + | *[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]] | ||
| + | ** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]] | ||
Revision as of 19:45, 4 February 2013
Back home: Lidstrom
Contents |
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Isolation
Manipulation
- Intro: Building with DNA (in progress)
- Digestion with Restriction Enzymes
- Gibson Assembly (In progress)
- Ligation (in progress)
- Overlap Extension PCR (in progress)
- Qiagen Buffer Recipes
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
Protein
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
