IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct

Super Parts	Prey Construct
Actual Part	T9002: Test Sensing Prey Construct
Sub Parts	F2620	intermediate_part	intermediate_part

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

use content from: IGEM:IMPERIAL/2006/project/parts/BBa_T9002

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Motivations

• This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
• This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
• This testing part allows us to investigate three parameters:
  • How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
  • How much AHL is present, by referring to the transfer function
  • How LuxR levels effect gene activation

Part interface

INPUTS OUTPUTS AHL GFP

Expected behavior and performances

(try to write your specifications in tables. Here are just 2 examples)

Interface specifiactions

Characteristics	Input 1	Input 2	Input 3	Output 1	Output 2
Range	[X1,Y1]	[X2,Y2]	[X3,Y3]	[X4,Y4]	[X5,Y5]
Tolerance	T1	T2	T3	T4	T5
Transfer function	n/a	n/a	n/a	insert image	insert image
Latency

System level specifications

Properties	Comments
Range
Transfer function
Latency
Robustness
Variability
properties 3
properties 4

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