IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct: Difference between revisions

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==Part interface==
==Part interface==


(describe your black-box approach, define properties of inputs/outputs)
{| border="0" width="100% align="center"
{| border="0" width="100% align="center"
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
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| input 1 || output 1
| N/A || GFP
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| input 2 ||rowspan = "2" | output 2
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| input 3
|}
|}
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[[Image:iGEM_IMPERIAL_Methodology_Specifications.png|right|350px]]
[[Image:iGEM_IMPERIAL_Methodology_Specifications.png|right|350px]]
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(don't forget to insert a relevant image of your black box.)


==Expected behavior and performances==
==Expected behavior and performances==

Revision as of 12:38, 21 October 2006

Super Parts Prey Construct
Actual Part
Sub Parts intermediate_part intermediate_part intermediate_part



use content from: IGEM:IMPERIAL/2006/project/parts/BBa_T9002

Use this template

Motivations

  • This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
  • This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
  • This testing part allows us to investigate three parameters:
    • How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
    • How much AHL is present, by referring to the transfer function
    • How LuxR levels effect gene activation

Part interface

INPUTS OUTPUTS
N/A GFP

Expected behavior and performances

(try to write your specifications in tables. Here are just 2 examples)

Interface specifiactions
Characteristics Input 1 Input 2 Input 3 Output 1 Output 2
Range [X1,Y1] [X2,Y2] [X3,Y3] [X4,Y4] [X5,Y5]
Tolerance T1 T2 T3 T4 T5
Transfer function n/a n/a n/a insert image insert image
Latency


System level specifications
Properties Comments
Range
Transfer function
Latency
Robustness
Variability
properties 3
properties 4

Open issues

  • list known issues
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