IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Killing Predator Construct/Implementation: Difference between revisions
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==Ligation Strategy== | ==Ligation Strategy== | ||
<u><font size="2">'''Ligating the AiiA Testing Construct:'''</font></u> | <u><font size="2">'''Ligating the AiiA Testing Construct:'''</font></u> | ||
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==WetLab work entries== | ==WetLab work entries== | ||
*The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct/Design design section for this part] for more information] | |||
*Parts J37024 and J37022 were not built due to time constraints. |
Latest revision as of 08:06, 30 October 2006
Super Parts | Predator Construct | |||
---|---|---|---|---|
Actual Part | AiiA Testing Construct <bbpart>J37022</bbpart> | |||
Sub Parts | <bbpart>R0010</bbpart> | <bbpart>B0034</bbpart> | <bbpart>J37023</bbpart> | <bbpart>B0015</bbpart> |
Ligation Strategy
Ligating the AiiA Testing Construct:
- More information on ligations can be found on the Ligation Page.
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
No | N/A | N/A | ||
Yes | Yes Note: This part was sequenced as a part of J37025 |
Primers used | ||
No | N/A | N/A |
WetLab work entries
- The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
- Parts J37024 and J37022 were not built due to time constraints.