IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Killing Predator Construct: Difference between revisions
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!height="25pt" width="80pt"|Actual Part | !height="25pt" width="80pt"|Actual Part | ||
!colspan="4"| [[Image:J37022a.png]] | !colspan="4"| AiiA Testing Construct <bbpart>J37022</bbpart>[[Image:J37022a.png]] | ||
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
Revision as of 13:46, 16 October 2006
| Super Parts | Predator Construct | |||
|---|---|---|---|---|
| Actual Part | AiiA Testing Construct <bbpart>J37022</bbpart>
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| Sub Parts | intermediate_part | intermediate_part | intermediate_part | intermediate_part |
Use content from: IGEM:IMPERIAL/2006/project/parts/BBa_J37022
AiiA forms an integral part of the biological oscillator acting as our predator in the Lotka-Volterra Predator-Prey Model. By testing and characterising this part individually, we can find the rate at which AiiA converts N-acyl homoserine lactone (AHL) into it's inactive form by cleaving the lactone ring (AHL-lactonase activity) at the ester bond.
Parameters to explore:
- Activity of AiiA
- Half-life of AiiA
Michaelis-menten kinetics would determine the activity of the enzyme, thus if we can characterise AiiA based upon it's activity (turnover rate) and half-life, the AiiA construct (part J37025) can be placed anywhere and easily integrated into more complex systems given it's transfer function defined by the enzyme parameters.
