G418

Experimental

Gentamycin analog, used to select for the KanMX marker in yeast.

Stock Solution

200mg/mL (1000X), stored at -20 C

Working Concentration

Working concentration depends on organism and purpose of antibiotic application.

• bacteria selection: 8-16 µg/ml

• plant cell maintenance: 10 µg/ml
• plant cell selection: 25-50 µg/ml

• mammalian cell maintenance: 200 µg/ml
• mammalian cell selection: 400 µg/ml
• yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.

Mode of Action

Irreversibly binds to 80S ribosomal subunit, disrupting proofreading.

Mode of resistance

neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.

Links

• FAQ on G418 [1]