Biomod/2011/Harvard/HarvarDNAnos:Results PC
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Photocleavage Test Results
Summary
- Photocleavage works for IDT internal photocleavable spacers!
- Applications of photo-cleavage can be applied for future use in DNA Origami Containers.
- See also: Photocleavage Protocol
Test 1
- Photocleavage Test 1: Using two strands with internal photocleavable spacers, directly from manufacturer-shipped tubes
- 10% TBE PAGE gel, 15 minute 365 nm UV exposure for "cleaved" strands
Test 2
- Photocleavage Test 2: using gel-purified uncleaved strands from Photocleavage Test 1
- We did this because there was some background photocleavage in Test 1
- We also wanted to test a longer exposure to UV light
- This experiment was successful: we saw nearly complete conversion to the cleaved product.
- 10% TBE PAGE gel, 1 hour 365 nm UV exposure for "cleaved" strands